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Effect of heat treatment on Germination of
Acacia longifolia and Dillwynia floribunda
G.Clark
University of Wollongong
Cheap College Papers on Heat treament on seeds
Abstract
Thegermination of two types of seeds Acacia longifolia and Dillwynia foribunda were studied after exposure to heat treatment. This was done by exposure to boiling water. Response to treatment was evaluated by final percentage of germination of each species after a time period.
There was a significant difference in response between the two species to the heat treatment.Acacia longifolia had higher germination percentage in the unheated group then in the group exposed to boiling water. While germination percentage in Dillwynia foribundawas much grater in the heated group, with very few seeds germinating in the unheated group.
Introduction
Bushfires are a regular part of many of Australia's ecosystems. Bushfires can be a major disturbing force, causing adult plant damage and possible death. Were adult plants are capable of resprouting after a fire no seedling re-generation may be need to maintain a population. However there may be some adult plant mortality during normal fire, or very high mortality, if intensity or frequency of fires increase . Therefore successful germination of seedlings will be needed to replace an adult population. ( Auld, T. D. and O'Connell 11)
The central hypothesis of the experiment was that Seeds from Acacia longifola and Dillwynia foribunda require a high temperature to the outer shell of the seed to break its dormancy and instigate the germination process in the two types of seeds. The null hypothesis was that no heat was required to instigate the germination of the two types of seeds.
These hypothesis were based on both seeds having a thick hard protective coating which leaves the seeds dormant to a force is attacked on them. This paper presents evidence that heat treatment to Dillwynia foribunda helps to increase germination, While in Acacia longifola there was no evidence that heat treatment helps increase germination.
Materials and Methods
Two species of seeds were studied, Acacia longifola and Dillwynia foribunda of which samples of each type of seeds were supplied.
The experiment was set up so that 10 seeds of each species with it's elaiosomes removed were placed in unheated water, then 10 of each species with elaiosomes removed were added to boiling water. This was repeated across five different groups along the beach.
The independent variable in this experiment is temperature, while the dependent variable is the seeds, the standardized treatment is the type of seed.
Experimental Treatments
The experimental treatment used in this experimentwas boiling water, the seeds were placed in the water just taken of the boil and then placed in the water for 0 minutes. The same volume of water were used for each group of seeds 00ml in group 5 this may have varied significantly over the five different groups.
The control treatment used in this experiment was unheated water, two groups of seeds were placed in water a room temperature and left for 0 minutes.
After 0 minutes the seeds were removed and placed in pitree dishes. They were then left for two weeks, there was no observation of the seeds during these two weeks.
Results
Table one lists the results that were gathered from the bench. There was a great deal of variation between groups, this has been attributed two error in experimental method and change in experimental conditions such as water temperature and time that the seeds were allowed to stay in the water as well as the natural variation within the species. 15 out of 50 Acacia seeds in the control treatment germinated with only 1 in 50 seeds germinating in the treated (heated) .of 50 Dillwynia germinated in control treatment while 7 in 50 germinated in the treated group. Germination percentage can be seen in fig. 1. The t-test for Acacia longifolai was .1 showing that there is a significant difference between the treated and untreated groups, shows that the likely hood of the means being the same is almost impossible. The t-test for Dillwynai florbunda was 1. also proving the likely hood of the two groups having the same mean as almost being impossible.
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